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Image Search Results
Journal: Journal of Cell Communication and Signaling
Article Title: Mesenchymal stromal cells of the bone marrow and natural killer cells: cell interactions and cross modulation
doi: 10.1007/s12079-018-0448-4
Figure Lengend Snippet: BM-MSCs were cultivated for 5 days either alone or in the presence of un-activated or PHA/IL-2-activated PBMCs. a Morphology of BM-MSCs (100× objective). b Cell viability measured by trypan blue exclusion assay. The values reported represent the means ± SEM of seven independent experiments, each performed in triplicate. ***p < 0.01 vs. MSCs; Paired Student’s t-test
Article Snippet:
Techniques: Trypan Blue Exclusion Assay
Journal: Journal of Cell Communication and Signaling
Article Title: Mesenchymal stromal cells of the bone marrow and natural killer cells: cell interactions and cross modulation
doi: 10.1007/s12079-018-0448-4
Figure Lengend Snippet: BM-MSCs were cultivated for 5 days either alone or in the presence of PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2-activated purified CD3+, CD14+ or CD56 cell fractions. Cell viability was measured by trypan blue exclusion assay. The percentage of viable BM-MSCs was calculated, and the values reported represent the means ± SEM of seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 vs. MSCs; Paired Student’s t-test
Article Snippet:
Techniques: Purification, Trypan Blue Exclusion Assay
Journal: Journal of Cell Communication and Signaling
Article Title: Mesenchymal stromal cells of the bone marrow and natural killer cells: cell interactions and cross modulation
doi: 10.1007/s12079-018-0448-4
Figure Lengend Snippet: BM-MSCs were cultivated for 5 days either alone or in the presence of un-activated or IL-2-, IL-12-, IL-15- or IL-21-activated NK cells. a Morphology of BM-MSCs (100× objective). b BM-MSC viability as measured by trypan blue exclusion assay. c BM-MSC death, as determined by 7 amino-actinomycin (7-AAD) staining assay. d Lactate Dehydrogenase (LDH) assay for measuring cellular toxicity. e Antibody mediated blocking assays of the interaction of CD112 and CD155 with CD226 during the LDH assay by using 10 μg/mL of monoclonal anti-human CD226 antibody (R&D Systems). The percentage of cytotoxicity was calculated as described in the Materials and Methods section, and the values reported represent the means ± SEM of seven independent experiments, each performed in triplicate. **p < 0.01 vs. MSCs; Paired Student’s t-test
Article Snippet:
Techniques: Trypan Blue Exclusion Assay, Staining, Lactate Dehydrogenase Assay, Blocking Assay